What is ICSI treatment? Differences with IVF, step-by-step process, and success rates

In the exciting realm of assisted reproduction, techniques are constantly advancing to offer new opportunities to those seeking to start a family. One of these techniques is ICSI (Intracytoplasmic Sperm Injection), a powerful tool to overcome male fertility challenges.
Are you eager to grasp the intricate details of what ICSI entails, how it is performed in the laboratory, and what its success rates are? In this blog, our specialist embryologist, Beatriz Lozano, will guide you through each step, explain the differences between ICSI and traditional IVF, and provide a comprehensive view of this cutting-edge technique that is revolutionizing the possibilities of conception.
Be prepared to explore the world of ICSI under the guidance of a fertility expert!
- 1. What is ICSI treatment?
- 2. Who is it Indicated for?
- 3. How is ICSI performed?
- 4. Differences between IVF and ICSI
- 5. ICSI Success Rates
- 6. How much does an ICSI treatment cost in Spain?
- 7. Our fertility treatments
- 8. Where can I find Vida Fertility centres in Spain?
- 9. What is the Reciprocal IVF and what does it consist of?
- 10. Our fertility treatments
- 11. The best fertility clinics in Spain
What is ICSI treatment?

Intracytoplasmic sperm injection (ICSI) is an in vitro fertilisation technique that involves introducing a sperm directly into the cytoplasm of the oocytes. One of the steps may require anaesthesia.
Who is it Indicated for?
The need for ICSI in assisted reproduction treatments primarily arises due to poor sperm quality. It is used to address male fertility issues such as oligozoospermia (low sperm concentration), teratozoospermia (morphological abnormalities), or astenozoospermia (reduced motility).
>> MALE INFERTILITY AND SEMEN PATHOLOGIES <<
However, in Vida Fertility laboratories, whenever patients agree, we use ICSI in our in vitro fertilisation treatments, as it is a technique that enhances pregnancy rates.
ICSI is also required in other circumstances to overcome various infertility causes such as:
- Implantation failures.
- Previous IVF treatments failures.
- Previous artificial insemination (AI) failures.
- Immunological infertility due to antisperm antibodies.
- Back-up microinjection in cases of failed fertilisation using conventional IVF.
- In Vitro maturation of the eggs.
- In case of preimplantation genetic testing (PGT).
- In case of using donor eggs or donor sperm.
- In case of using frozen eggs.
How is ICSI performed?
The procedure involves the following steps:
- Ovarian Stimulation: The patient undergoes hormonal therapy to stimulate her growing follicules and optimise fertilisation.
- Egg Retrieval: Under anesthesia, mature eggs are retrieved from the ovaries through surgical aspiration.
- Oocyte Denudation or Decumulation: The surrounding tissue of the collected eggs is removed, eliminating cumulus cells to assess the maturity status of the oocytes.
- Sperm Preparation: Seminal capacitation for an enhanced fertilisation capacity.
- ICSI: A sperm is selected and injected into the egg.
- Embryo Culture: Embryos develop in an incubator under optimal conditions.
- Endometrial preparation: The endometrium is ready to welcome a pregnancy.
- Embryo Transfer: A catheter is inserted into the vagina to place embryos in the uterus.
>> All You Need to Know About Embryo Transfer <<
- Embryo Freezing: Untransferred good quality embryos are frozen and stored for future use.

Differences between IVF and ICSI
The distinctions between In Vitro Fertilisation (IVF) and Intracytoplasmic Sperm Injection (ICSI) are crucial in the world of assisted reproduction.
- In conventional IVF, eggs and sperm are placed together in a culture dish, allowing fertilisation to occur naturally.
- On the other hand, with ICSI, a single sperm is selected and microinjected directly into the egg, ensuring a higher probability of fertilisation in cases of male fertility issues.
ICSI becomes the preferred choice when there are problems with sperm quality or quantity. Laboratory experts carefully assess each case to determine the most suitable technique. While IVF is a highly effective process, ICSI maximizes success chances in more complex situations.
Our assisted reproduction specialists are committed to providing you with detailed information about the differences between these techniques, ensuring that every patient receives the most appropriate treatment for their unique situation.

ICSI Success Rates
Thanks to the precision and control provided by ICSI in selecting and microinjecting a sperm into the egg, fertilisation is optimised, succeeding where conventional IVF might not. This technique has become an essential resource for couples facing fertility difficulties related to male causes.
Experts in the field of assisted reproduction, including Beatriz Lozano, recognise that ICSI has revolutionised how we address male infertility, giving hope to couples striving to build a family.
How much does an ICSI treatment cost in Spain?
The price of an ICSI treatment (Intracytoplasmic Sperm Injection) can vary depending on each patient’s individual circumstances. At Vida Fertility, we understand that no two cases are the same: every woman, every couple, and every fertility journey is unique.
That is why, before talking about the cost of an ICSI treatment, the most important step is to carry out a complete fertility assessment. This allows us to design a treatment plan that is fully tailored to your needs. Based on this, we can provide you with a personalised and realistic quote, ensuring it includes the tests and techniques that are best suited to your situation.

Where can I find Vida Fertility centres in Spain?
📍 C/ Palermo 15, Hortaleza, 28043 Madrid
📞 Phone number: +34 919 29 83 23
🕒 Opening hours: Monday to Friday from 8am to 7pm
📍 Av. Óscar Esplá 1, Bajo, 03008 Alicante
📞 Phone number: +34 919 29 83 23
🕒 Opening hours: Monday to Friday from 8am to 7pm
The step-by-step of the reciprocal IVF process in Spain

The Reciprocal IVF or Ropa Method has gained popularity in Spain not only because it allows both mothers to physically participate in the process of conceiving their child, but also because it is backed by progressive legislation that recognises and facilitates various family configurations.
We spoke to Dr. Katharina Spies about the process of the ropa method in Spain, current laws and how to opt for this treatment if you come from outside Spain

What is the Reciprocal IVF and what does it consist of?
The reciprocal IVF or, ROPA method, or Reception of Oocytes from the same Couple, is an assisted reproduction technique specially designed for couples of women who wish to actively share the process of motherhood.
One of the women provides the eggs and the other gestate the baby, strengthening the emotional and biological bond of both with their future child.
Vida Fertility Events
At Vida Fertility, we believe that feeling informed is the first step towards a more confident fertility journey.
Through our events, talks and private consultations, we help patients understand their options, ask questions and receive personalised guidance from our fertility specialists in Spain.
Discover our upcoming events and meet the Vida Fertility team.
The best fertility clinics in Spain
📍 C/ Palermo 15, Hortaleza, 28043 Madrid
📞 Phone number: +34 919 29 83 23
🕒 Opening hours: Monday to Friday from 8am to 7pm
📍 Av. Óscar Esplá 1, Bajo, 03008 Alicante
📞 Phone number: +34 919 29 83 23
🕒 Opening hours: Monday to Friday from 8am to 7pm
ℹ️ The information published on Vida Fertility is reviewed by our fertility specialists and medical team, following up-to-date scientific criteria and evidence-based medicine. Our team is a member at scientific societies such as SEF, ESHRE and SEGO.






















